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cytokine protein microarray analysis cytokine protein levels  (RayBiotech inc)


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    RayBiotech inc cytokine protein microarray analysis cytokine protein levels
    Cytokine Protein Microarray Analysis Cytokine Protein Levels, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+microarray/Mouse+Cytokine+Array+GS1000/pm42680000-156-0-11
    Average 94 stars, based on 1 article reviews
    cytokine protein microarray analysis cytokine protein levels - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Protein Array:

    Article Title: Osteoarthritic synovial fibroblasts drive inflammatory responses and impair chondrocyte function in response to a fibronectin matrikine
    Article Snippet: .. The C5 human cytokine protein array (RayBiotech, AAH-CYT-5–2) was used to evaluate the secreted proteins found in the CM. ..

    Article Title: Metabolic dysregulation-triggered neutrophil extracellular traps exacerbate acute liver failure.
    Article Snippet: Edited by Wilfried Ellmeier Acute liver failure (ALF) is an acute liver disease with a high mortality rate in clinical practice, characterized histologically by extensive hepatocellular necrosis and massive neutrophil infiltration.. However, the role of these abnormally infiltrating neutrophils during ALF development is unclear.. Here, in an ALF mouse model, metabolites were identified that promote the formation of neutrophil extracellular traps (NETs) in the liver, subsequently influencing macrophage differentiation and disease progression.

    Microarray:

    Article Title: Mixed Th1/Th2/Th17 Responses Induced by Plant Oil Adjuvant-Based B. bronchiseptica Vaccine in Mice, with Mechanisms Unraveled by RNA-Seq, 16S rRNA and Metabolomics.
    Article Snippet: Then, 50 μL of stop solution Vaccines 2024, 12, 1182 5 of 26 (2 M H2SO4) was added to each well and the optical density (OD) of the plate was read at 450 nm with a microplate reader (Thermo-Multiskan, Shanghai, China). .. The serum samples were analyzed by a glass-based and sandwich-based antibody microarray that allowed the quantitative analysis of 18 different cytokines (Mouse Cytokine Array GS1, RayBiotech, Inc., Guangzhou, China). ..

    Article Title: Metabolic dysregulation-triggered neutrophil extracellular traps exacerbate acute liver failure.
    Article Snippet: Edited by Wilfried Ellmeier Acute liver failure (ALF) is an acute liver disease with a high mortality rate in clinical practice, characterized histologically by extensive hepatocellular necrosis and massive neutrophil infiltration.. However, the role of these abnormally infiltrating neutrophils during ALF development is unclear.. Here, in an ALF mouse model, metabolites were identified that promote the formation of neutrophil extracellular traps (NETs) in the liver, subsequently influencing macrophage differentiation and disease progression.

    Article Title: The monoacylglycerol acyltransferase 2 inhibitor VB-85387 reduces MASLD.
    Article Snippet: pIB and total IB (Abcam, #ab279827); pJAK1 and total JAK1 (Fortis Life Sciences, E172-003); IL-6 (Abcam, ab222503); IL-4 (Abcam, #ab100710); CRP (Abcam, #ab222511); IL-1 (Abcam #ab197742); IFN (Abcam #ab252363); COL1A1 (Abcam, #ab210579); COL3A1 (LS Bio, #LS-F25411); SMA (LS-F21849) kits were used for analysis. .. Jo urn al Pr e-p roo f 12 Apoptosis protein microarray analysis Apoptosis signaling was assessed using the RayBiotech Mouse Apoptosis Signaling Pathway Phosphorylation Array C1 (#AAM-APOSIG-1-2; Peachtree Corners, GA) according to the manufacturer’s protocol. ..

    Article Title: The monoacylglycerol acyltransferase 2 inhibitor VB-85387 reduces MASLD.
    Article Snippet: .. Cytokine protein microarray analysis Cytokine protein levels were measured using the RayBiotech Mouse Cytokine Array GS1000 (#GSMCAA-1000) according to the manufacturer’s instructions. ..

    Ab Array:

    Article Title: Metabolic dysregulation-triggered neutrophil extracellular traps exacerbate acute liver failure.
    Article Snippet: Edited by Wilfried Ellmeier Acute liver failure (ALF) is an acute liver disease with a high mortality rate in clinical practice, characterized histologically by extensive hepatocellular necrosis and massive neutrophil infiltration.. However, the role of these abnormally infiltrating neutrophils during ALF development is unclear.. Here, in an ALF mouse model, metabolites were identified that promote the formation of neutrophil extracellular traps (NETs) in the liver, subsequently influencing macrophage differentiation and disease progression.

    Phospho-proteomics:

    Article Title: The monoacylglycerol acyltransferase 2 inhibitor VB-85387 reduces MASLD.
    Article Snippet: pIB and total IB (Abcam, #ab279827); pJAK1 and total JAK1 (Fortis Life Sciences, E172-003); IL-6 (Abcam, ab222503); IL-4 (Abcam, #ab100710); CRP (Abcam, #ab222511); IL-1 (Abcam #ab197742); IFN (Abcam #ab252363); COL1A1 (Abcam, #ab210579); COL3A1 (LS Bio, #LS-F25411); SMA (LS-F21849) kits were used for analysis. .. Jo urn al Pr e-p roo f 12 Apoptosis protein microarray analysis Apoptosis signaling was assessed using the RayBiotech Mouse Apoptosis Signaling Pathway Phosphorylation Array C1 (#AAM-APOSIG-1-2; Peachtree Corners, GA) according to the manufacturer’s protocol. ..

    Article Title: PlexinD1 is a driver and a therapeutic target in advanced prostate cancer
    Article Snippet: Human HGF ELISA Kit , RayBiotech , ELH-HGF. .. Human RTK Phosphorylation Array , RayBiotech , AAJ-PRTK-1-2. .. NE-PER Nuclear and Cytoplasmic Extraction Reagents , Thermo Fisher Scientific , 78833.

    Enzyme-linked Immunosorbent Assay:

    Article Title: PlexinD1 is a driver and a therapeutic target in advanced prostate cancer
    Article Snippet: Duolink In Situ Red Starter Kit , Sigma-Aldrich , DUO92101. .. Human HGF ELISA Kit , RayBiotech , ELH-HGF. .. Human RTK Phosphorylation Array , RayBiotech , AAJ-PRTK-1-2.

    Cell Culture:

    Article Title: Decoded cardiopoietic cell secretome linkage to heart repair biosignature.
    Article Snippet: .. To this end, cardiopoietic cells manufactured from bone marrow MSCs of ischemic heart failure patients undergoing clinical testing were cultured.11,19 Conditioned media was collected and scanned with the RayBiotech Human L507 Array protein chip. ..



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    Image Search Results


    Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.

    Journal: Journal of Virology

    Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

    doi: 10.1128/jvi.00478-25

    Figure Lengend Snippet: Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.

    Article Snippet: Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on PATH Protein Microarray Slides (GraceBio-Labs, Oregon, USA).

    Techniques: Microarray, Labeling, Fluorescence, Infection

    High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.

    Journal: Journal of Virology

    Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

    doi: 10.1128/jvi.00478-25

    Figure Lengend Snippet: High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.

    Article Snippet: Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on PATH Protein Microarray Slides (GraceBio-Labs, Oregon, USA).

    Techniques: High Throughput Screening Assay, Infection, Microarray, Virus